Microorganisms for the recombinant production of resveratrol and other flavonoids
AssigneeRegents of the University of Minnesota
InventorsClaudia Schmidt-Dannert, Kevin Watts
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Abstract
The invention provides methods and materials related to producing flavonoids as well as other organic compounds. For example, the invention provides isolated nucleic acids, polypeptides, host cells, and methods and materials for producing flavonoids and other organic compounds.
Claims15
An E. coli microorganism comprising an exogenous nucleic acid encoding a naturally occurring polypeptide having coumaroyl-CoA-ligase activity and an exogenous nucleic acid encoding a naturally occurring polypeptide having stilbene synthase activity, wherein the polypeptide having coumaroyl-CoA-ligase activity is a plant polypeptide, a Streptomyces polypeptide, a Pseudomonas polypeptide, or a Rhodobacter polypeptide, wherein the polypeptide having stilbene synthase activity is an Arachis hypogaea polypeptide, a Vitis vinifera polypeptide, a Rheum tataricum polypeptide, a Psilotum nudum polypeptide, or a Pinus sylvestris polypeptide, and wherein a culture comprising a plurality of said microorganisms produces and secretes at least 20 mg/L of a flavonoid compound.
An E. coli microorganism comprising an exogenous nucleic acid encoding a polypeptide having coumaroyl-CoA-ligase activity, wherein said polypeptide having coumaroyl-CoA-ligase activity is an Arabidopsis thaliana, Medicago truncatula, Arachis hypogaea, Streptomyces coelicolor, Streptomyces avermitilis, Pseudomonas spp. or Rhodobacter spp. polypeptide, and an exogenous nucleic acid encoding a polypeptide having stilbene synthase activity, wherein said polypeptide having stilbene synthase activity is an Arachis hypogaea, Vitis vinifera, Rheum tataricum, Psilotum nudum, or Pinus sylvestris polypeptide, and wherein a culture comprising a plurality of said microorganisms produces and secretes at least 20 mg/L of resveratrol.
An E. coli microorganism comprising exogenous nucleic acids encoding polypeptides having phenol-type CoA-ligase activity and stilbene synthase activity, wherein said polypeptide having phenol-type CoA-ligase activity comprises the sequence set forth in SEQ ID NO:2, wherein said polypeptide having stilbene synthase activity comprises the sequence set forth in SEQ ID NO:6, and wherein said microorganism produces and secretes a flavonoid compound.
An E. coli microorganism comprising an exogenous nucleic acid encoding a naturally occurring polypeptide having coumaroyl-CoA-ligase activity, an exogenous nucleic acid encoding a naturally occurring polypeptide having stilbene synthase activity, and an exogenous nucleic acid encoding a naturally occurring polypeptide having tyrosine ammonia lyase activity, wherein the polypeptide having coumaroyl-CoA-ligase activity is a plant polypeptide, a Streptomyces polypeptide, a Pseudomonas polypeptide, or a Rhodobacter polypeptide, wherein the polypeptide having stilbene synthase activity is an Arachis hypogaea polypeptide, a Vitis vinifera polypeptide, a Rheum tataricum polypeptide, a Psilotum nudum polypeptide, or a Pinus sylvestris polypeptide, wherein the polypeptide having tyrosine ammonia lyase activity is a Rhodobacter capsulatus or Rhodobacter sphaeroides polypeptide, and wherein a culture comprising a plurality of said microorganisms produces and secretes at least 20 mg/L of resveratrol.
Dependencies are inferred from the claim text.