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US 7,604,961 B2Granted
VP1 of foot-and-mouth disease virus
AssigneeAcademia Sinica
InventorsShu-Mei Liang, Jeng-Hwan Wang, Jeng-Jer Shieh
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Abstract
A pure, water-soluble polypeptide containing one or more monomers of a VP1 protein of a foot-and-mouth disease virus; or a pure, water-insoluble polypeptide comprising two or more monomers of a VP1 protein of a foot-and-mouth disease virus. Also disclosed are a vaccine containing the polypeptide, a method of producing the polypeptide, and a method of inducing an immune response in a subject by administering to the subject an effective amount of the polypeptide.
Claims4
A method of producing a water-soluble polypeptide, the method comprising:
- unfolding a water-insoluble polypeptide that contains one or more monomers of a VP1 protein of a foot-and-mouth disease virus in a first solution containing urea, and
- refolding the polypeptide, thereby producing a water-soluble polypeptide, wherein the refolding step includes treating the polypeptide in a second solution containing sodium dodecyl sulfate followed by gel filtration column chromatography.
A method of producing a polypeptide, the method comprising:
- expressing in a cell a water-insoluble polypeptide that contains two or more monomers of a VP1 protein of a foot-and-mouth disease virus,
- collecting the polypeptide from the cell,
- unfolding the polypeptide in a first solution containing urea, and
- refolding the polypeptide, thereby producing a water-soluble polypeptide, wherein the refolding step includes treating the polypeptide in a second solution containing sodium dodecyl sulfate followed by gel filtration column chromatography.
1 more element
Dependencies are inferred from the claim text.