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US 7,604,958 B2Granted
Synthetic mammalian α-N-acetylglucosaminidase and genetic sequences encoding same
AssigneeCentral Northern Adelaide Health Service
InventorsJohn Joseph Hopwood, Hamish Steele Scott, Birgit Weber, Lianne Blanch, Donald Stewart Anson
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Abstract
The present invention relates generally to mammalian α-N-acetyglucosaminidase and to genetic sequences encoding same and to their use in the investigation, diagnosis and treatment of subjects suspected of or suffering from α-N-acetyglucosaminidase deficiency.
Claims5
A method for the production of α-N-acetylglucosaminidase, said method comprising:
- (i) transfecting mammalian cells with an expression vector comprising a DNA molecule encoding α-N-acetylglucosaminidase having the amino acid sequence of SEQ ID NO:2 or fragment thereof comprising amino acids 24-743 of SEQ ID NO:2 fused to a signal sequence;
- (ii) culturing the transfected cells, whereby the α-N-acetylglucosaminidase protein having the amino acid sequence of SEQ ID NO:2 or fragment thereof comprising amino acids 24-743 of SEQ ID NO:2 fused to a signal sequence is produced and α-N-acetylglucosaminidase protein secreted into the medium;
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- (iii) recovering the α-N-acetylglucosaminidase protein, from the medium.
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Dependencies are inferred from the claim text.