Bioamplification for microbial sensor signal transduction
AssigneesIowa State University Research Foundation, Inc., The United States of America as represented by the Department of Agriculture/Cooperative State Research Education and Extension Service (USDA/CSREES)
InventorsMarc D. Porter, Betsy Jean Yakes, Robert J. Lipert, John P. Bannantine
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Abstract
A process of detection of the causative agent of Johne's disease (Mycobacterium avium subsp. paratuberculosis) (MAP) by detecting shedding of surface protein of MAP. A preferred way is use of surface enhanced Raman Spectroscopy. The system of detecting MAP shedding of protein provides early detection and diagnosis, and therefore allows early treatment for Johne's disease in ruminant animals.
Claims8
A process of detection of Mycobacterium avium subsp. paratuberculosis (MAP) to diagnose Johne's disease in ruminants, comprising:
- collecting a bacteria sample from the ruminant to be tested;
- heat treating the bacteria sample to allow any MAP present to shed protein from their surface;
- binding the shed protein to a monoclonal antibody specific for MAP or MAP surface protein to provide bound particles to allow detection of the MAP protein;
- and
- detecting the presence of MAP or MAP shed protein by immunoassaying the bound particles.
A heterogeneous sandwich immunoassay for use in SERS detection of MAP, comprising:
- an immunogold substrate;
- a MAP antigen attached to said substrate;
- an Extrinsic RL attached to said MAP antigen.
A kit for detection of Johne's disease, comprising:
- a Raman active reporter molecule;
- a substrate for a MAP antigen to attach;
- a surface enhancing particle reagent;
- and
- instructions of using the kit in SERS detection of surface shedding protein of MAP.
Dependencies are inferred from the claim text.