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US 7,604,942 B2Granted
Systems and methods of analyzing nucleic acid polymers and related components
AssigneeZS Genetics, Inc.
InventorWilliam Roy Glover, III
Life of this patent
Estimating the term…
Abstract
Systems and methods of identifying, sequencing and/or detecting nucleic acid polymers, as well as related components (e.g., substrates, software and the like) are disclosed.
Claims18
A method of detecting the presence, quantifying and/or identifying a nucleic acid polymer,
- comprising forming a complementary strand of the nucleic acid polymer,
- attaching the complementary strand and, optionally, the nucleic acid polymer to a substrate, and
- detecting the presence, quantifying and/or identifying the complementary strand and/or the nucleic acid polymer using a particle beam,
- wherein the step of identifying comprises measuring the length or determining at least a partial sequence of the complementary strand and/or the nucleic acid polymer.
1 more element
A method of detecting the presence, quantifying and/or identifying a nucleic acid polymer,
- comprising forming a complementary strand of the nucleic acid polymer,
- attaching the complementary strand and, optionally, the nucleic acid polymer to a substrate, and
- detecting the presence, quantifying and/or identifying the complementary strand and/or the nucleic acid polymer using a particle beam,
- wherein the nucleic acid polymer and/or its complementary strand is formed by a nucleic acid polymerase enzyme.
1 more element
A method of detecting the presence, quantifying and/or identifying a nucleic acid polymer,
- comprising forming a complementary strand of the nucleic acid polymer,
- attaching the complementary strand and, optionally, the nucleic acid polymer to a substrate, and
- detecting the presence, quantifying and/or identifying the complementary strand and/or the nucleic acid polymer using a particle beam,
- wherein the nucleotides of the nucleic acid polymer and/or the complementary strand are modified to include labels, and
- wherein the labels comprise one or more atoms.
2 more elements
A method of detecting the presence, quantifying and/or identifying a nucleic acid polymer,
- comprising forming a complementary strand of the nucleic acid polymer,
- attaching the complementary strand and, optionally, the nucleic acid polymer to a substrate, and
- detecting the presence, quantifying and/or identifying the complementary strand and/or the nucleic acid polymer using a particle beam,
- wherein the nucleotides of the nucleic acid polymer and/or the complementary strand are modified to include labels,
- wherein the labels are specific for each type of nucleotide, and
- wherein nucleotide specific labels are bonded to the nucleotides of the nucleic acid polymer and/or the complementary strand after formation of the nucleic acid polymer and/or the complementary strand.
3 more elements
A method of detecting the presence, quantifying and/or identifying a nucleic acid polymer,
- comprising forming a complementary strand of the nucleic acid polymer,
- attaching the complementary strand and, optionally, the nucleic acid polymer to a substrate, and
- detecting the presence, quantifying and/or identifying the complementary strand and/or the nucleic acid polymer using a particle beam,
- wherein the nucleic acid polymer and/or the complementary strand are substantially straightened by fluid flow prior to detecting the sequence,
- wherein the fluid comprises one or more liquids, gases, phases or a combination thereof, and/or
- wherein the nucleic acid polymer and/or the complementary strand are attached to a substrate and straightened by hybridization in a fluid flow to oligonucleotides that are attached to the substrate.
3 more elements
A method of detecting the presence, quantifying and/or identifying a nucleic acid polymer,
- comprising forming a complementary strand of the nucleic acid polymer,
- attaching the complementary strand and, optionally, the nucleic acid polymer to a substrate, and
- detecting the presence, quantifying and/or identifying the complementary strand and/or the nucleic acid polymer using a particle beam,
- wherein the step of identifying the nucleotides in the nucleic acid polymer and/or its complementary strand comprises interpreting changes in the particle beam resulting from interactions with the nucleotides to detect the nucleotides in the nucleic acid polymer and/or its complementary strand, whereby the presence, length and/or sequence of the nucleic acid polymer is determined, and
- wherein the changes in the particle beam are changes in absorbance, reflection, deflection, energy or direction, and/or a spatial pattern.
2 more elements
Dependencies are inferred from the claim text.