Back to results
US 7,604,941 B2Granted
Nucleotide sequencing via repetitive single molecule hybridization
OwnerJoseph M. Jacobsoninventor
Life of this patent
Estimating the term…
Abstract
Methods of obtaining sequence information about target oligonucleotides by repetitive single molecule hybridization are disclosed. The methods include exposing a target oligonucleotide to one or more copies of a test oligonucleotide; measuring hybridization; dehybridizing the test oligonucleotide; and repeating until the information content from the hybridization trials equals or exceeds the information content of the target oligonucleotide.
Claims26
A method of analyzing nucleic acids comprising:
- (a) hybridizing one or more copies of a test oligonucleotide comprising at least two informative region sequences to a single molecule of target oligonucleotides under conditions permitting hybridization of the test oligonucleotide to a perfectly complementary sequence;
- (b) measuring a signal of said hybridization of the test oligonucleotide to the target oligonucleotide, thereby completing a first hybridization trial;
- (c) dehybridizing the test oligonucleotide;
- (d) exposing the target oligonucleotide to one or more copies of at least one additional test oligonucleotide, said additional test oligonucleotide comprising at least two informative regions;
- (e) measuring a signal from the hybridization of the additional test oligonucleotide to the target oligonucleotide, thereby completing a further hybridization trial;
- (f) repeating steps c) to e) until the information content from the hybridization trials exceeds the information content of the target oligonucleotide;
- and
- (g) analyzing said signals.
5 more elements
A method of analyzing nucleic acids comprising:
- (a) hybridizing one or more copies of a test oligonucleotide to a single molecule of target oligonucleotides under conditions permitting hybridization of the test oligonucleotide to a perfectly complementary sequence;
- (b) measuring a signal of said hybridization of the test oligonucleotide to the target oligonucleotide, thereby completing a first hybridization trial;
- (c) dehybridizing the test oligonucleotide;
- (d) exposing the target oligonucleotide to one or more copies of at least one additional test oligonucleotide;
- (e) measuring a signal from the hybridization of the additional test oligonucleotide to the target oligonucleotide, thereby completing a further hybridization trial;
- (f) repeating steps c) to e) until the information content from the hybridization trials exceeds the information content of the target oligonucleotide;
- and
- (g) analyzing said signals;
- and
- (h) analyzing an absence of said signals to eliminate oligonucleotide test members.
7 more elements
Dependencies are inferred from the claim text.