Method for sorting tumor cells
AssigneeOncoscience AG
InventorsFerdinand Bach, Joseph Fischer
Life of this patent
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Abstract
The present invention relates to a method for storing cells, wherein the cells are stored in a composition comprising a base nutritive medium and liposomes at temperatures in the range of −196° C. to 37° C., characterized in that the liposomes comprise one or more sterols and that the cells are tumor cells. More specifically, the invention relates to simple and effective methods for storing tumor cells, wherein the RNA and/or DNA in the cells is essentially not degraded, such that the RNA and/or DNA can be analyzed after storage.
Claims30
A method for storing tumor cells in a composition, said method comprising:
- providing a composition and tumor cells, wherein said composition is enriched with oxygen and comprises a base nutritive medium and liposomes that comprise at least 0.00475 g/L cholesterol;
- and
- storing said tumor cells in said composition at temperatures in the range of −196° C. to 0° C.
A method for freezing cells in a composition, said method comprising:
- providing a composition and cells, wherein said composition is enriched with oxygen and comprises a base nutritive medium and liposomes that comprise at least 0.00475 g/L cholesterol;
- and
- freezing said cells in said composition.
A composition comprising tumor cells, a base nutritive medium, and liposomes, wherein said liposomes comprise at least 0.00475 g/L cholesterol and said composition is enriched with oxygen.
A library of tumor biopsies comprising multiple compositions wherein each composition comprises tumor cells, a base nutritive medium, and liposomes, said liposomes comprise at least 0.00475 g/L cholesterol, said composition is enriched with oxygen, and each composition is stored in a separate container.
A method of preparing a composition for storing cells, said method comprising:
- preparing a base nutritive medium comprising physiologically compatible concentrations of water-soluble or dispersible nutrients and physiological salts;
- preparing nanoparticles, which are liposomes comprising at least 0.00475 g/L cholesterol, fatty acids, and cellular growth factors;
- and
- emulsifying said base nutritive medium and said nanoparticles to form a two phase composition, wherein said composition has an osmolality of at least about 300 mOsM/kg, does not contain a cryoprotective agent and is enriched with oxygen by bubbling oxygen through the composition until saturation.
Dependencies are inferred from the claim text.