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US 7,604,932 B2Granted
Assay for tissue factor in a sample
AssigneeHaematologic Technologies, Inc.
InventorsRichard J. Jenny, Saulius Butenas
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Abstract
The invention provides assays for detecting and quantitating tissue factor and factor VIIa in simple and complex biological systems. The assays are performed by detecting and/or measuring the tissue factor cofactor activity and factor VIIa enzymatic activity using aminonapthalene-based fluorogenic substrates.
Claims12
A method for determining the concentration of tissue factor (TF) in a sample suspected to contain TF, comprising:
- (a) combining the sample and a molar excess of factor VIIa (fVIIa) compared to the moles of TF in the sample to produce a TF/fVIIa enzyme complex;
- (b) detecting the enzymatic activity of the complex using a fluorogenic or chromogenic substrate;
- (c) generating numerical values correlated with the enzymatic activity of the sample;
- and (d) comparing the numerical values with a standard curve of TF-dependent enzymatic activity, wherein the standard curve is generated by quantifying TF-dependent enzymatic activity of the TF/fVIIa complex in samples with known concentrations of TF.
1 more element
Dependencies are inferred from the claim text.