Method of making stably preserved microspheres in lower alcohol solvent
AssigneeHitachi Software Engineering Co., Ltd.
InventorsHisashi Ukawa, Akio Yamane
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Abstract
Active carboxylic acid ester groups are coupled on the surfaces of microspheres so as to reduce protocols for microsphere processing, control side reactions, and stably preserve beads containing active carboxylic acid ester groups. Further, microspheres labeled with at least one fluorescent dye cage in the microspheres, and the microspheres are preserved in lower alcohol.
Claims9
A method of stabilizing activated carboxyl groups on microspheres, comprising the steps of:
- activating carboxyl groups thereby forming active esters;
- covering microspheres with the active esters;
- filling the covered microspheres with at least one fluorescent dye;
- and
- storing the microspheres in a solvent which consists of lower alcohol,
- wherein the lower alcohol is selected from the group consisting of 1-butanol, methanol, ethanol, n-propanol, and isopropanol.
The method according to 1, wherein the carboxyl group is activated with at least one of a 2,3,5,8-tetrafluorophenol ester group and a 4-sulfo-2,3,5,6-tetrafluorophenol ester group.
The method according to 1, further comprising:
- repeatedly suspending the solvent with phosphate buffered saline (PBS) as a coupling buffer thereby coupling proteins onto surfaces of the microspheres stored in the solvent.
The method according to 6, wherein the PBS is at pH 7.4.
The method according to 1, further comprising:
- repeatedly using a phosphate buffer solution as a coupling buffer thereby coupling nucleic acids onto surfaces of the microspheres stored in the solvent.
The method according to 8, wherein the phosphate buffer solution is 0.1 M phosphate buffer at pH 8.0.
Dependencies are inferred from the claim text.