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US 7,604,824 B2Granted
Method for extraction lectin from Chaenomeles lagenaria, medical composition for inhibiting tumor growth, biological reagent for detecting glycophorin A on blood and blood typing kit
AssigneeTzu Chi Buddhist General Hospital
InventorKuo-Liang Yang
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Abstract
A Chaenomeles lagenaria extract having a lectin specific for glycophorin A and method for extracting the same are proposed. The extract is prepared by homogenizing seeds of Chaenomeles lagenaria with a homogenizing agent into a mixture, and the mixture is further processed to produce the extract both effective in inhibiting tumor growth and applicable to a blood typing test.
Claims7
A method for extracting a Chaenomeles lagenaria extract containing lectin specific for N-acetyl-D-galactosamine residues of O-glycosidically linked oligosaccharide of glycophorin A, comprising the steps of:
- homogenizing Chaenomeles lagenaria seeds with a homogenizing agent comprising phosphate buffered saline and glycine to produce a mixture;
- subjecting the mixture to centrifugation to separate the mixture into a pellet and supernatant;
- and collecting and filtering the supernatant to obtain the Chaenomeles lagenaria extract.
Dependencies are inferred from the claim text.