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US 7,604,798 B2Granted
Methods and compositions for importing nucleic acids into cell nuclei
AssigneeNorthwestern University
InventorDavid A. Dean
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Abstract
The present invention relates to methods and compositions for importing DNA into the nuclei of a specific cell type. In particular, the present invention provides methods and compositions for specifically targeting the nuclei of pulmonary cells.
Claims5
A method for tissue specific nuclear import, comprising:
- a) providing
- i) a construct comprising a cell-specific nuclear targeting signal operably linked to a nucleic acid of interest, wherein said cell-specific nuclear targeting signal comprises at least nucleotides 149 to 337 of the surfactant protein C(SPC) promoter region;
- shown in SEQ ID NO:1 that functions as a nuclear targeting signal;
- and
- ii) a non-dividing mouse or human alveolar type II cell in vitro;
- and
- b) contacting said cell with said construct under conditions such that said nucleic acid of interest is expressed in the nucleus of said cell.
4 more elements
Dependencies are inferred from the claim text.