Fluorescent light tomography
AssigneeXenogen Corporation
InventorsBradley W. Rice, Chaincy Kuo, Daniel G. Stearns, Heng Xu
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Abstract
Described herein are systems and methods for obtaining a three-dimensional (3D) representation of the distribution of fluorescent probes inside a sample, such as a mammal. Using a) fluorescent light emission data from one or more images, b) a surface representation of the mammal, and c) computer-implemented photon propagation models, the systems and methods produce a 3D representation of the fluorescent probe distribution in the mammal. The distribution may indicate—in 3D—the location, size, and/or brightness or concentration of one or more fluorescent probes in the mammal.
Claims36
A method for obtaining a light distribution located inside an animal, the method comprising:
- obtaining one or more fluorescent images of at least a portion of the animal;
- obtaining a three dimensional representation of a surface portion of the animal;
- mapping fluorescent image data from the one or more fluorescent images to the three dimensional representation of the surface portion of the animal to create fluorescent light emission data from the surface portion of the animal;
- and
- determining a three-dimensional representation of a fluorescent probe distribution internal to the animal with a processor using the fluorescent light emission data from the surface portion of the animal.
A method for obtaining a three-dimensional representation of a fluorescent probe distribution located inside a mammal, the method comprising:
- obtaining a first fluorescent image of at least a portion of the mammal;
- obtaining a second fluorescent image of at least a portion of the mammal,
- where the first fluorescent image includes a first trans-illumination position for an excitation light source relative to a camera and the second fluorescent image includes a second trans-illumination position for the excitation light source relative to the camera;
- obtaining a surface representation of a surface portion of the mammal;
- mapping fluorescent image data from the fluorescent image to the surface portion of the mammal to create fluorescent light emission data from the surface portion of the mammal;
- and
- determining a three-dimensional representation of the fluorescent probe distribution internal to the mammal with a processor using the fluorescent light emission data from the surface portion of the mammal.
A method for obtaining a three-dimensional representation of a fluorescent probe distribution located inside a mammal, the method comprising:
- obtaining a fluorescent image of at least a portion of the mammal;
- obtaining a surface representation of a surface portion of the mammal;
- mapping fluorescent image data from the fluorescent image to the surface portion of the mammal to create fluorescent light emission data from the surface portion of the mammal;
- determining autofluoresence data in the mammal;
- altering the fluorescent light emission data with the autofluorescence data;
- and
- determining a three-dimensional representation of the fluorescent probe distribution internal to the mammal with a processor using the fluorescent light emission data from the surface portion of the mammal.
Logic encoded in one or more tangible media for executable instructions and, when executed, operable to obtain a representation of a fluorescent probe distribution located inside a mammal, the logic including:
- executable instructions for obtaining one or more fluorescent images of at least a portion of the animal;
- executable instructions for obtaining a three dimensional representation of a surface portion of the animal;
- executable instructions for mapping fluorescent image data from the one or more fluorescent images to the three dimensional representation of the surface portion of the animal to create fluorescent light emission data from the surface portion of the animal;
- and
- executable instructions for determining a three-dimensional representation of the fluorescent probe distribution internal to the animal using the fluorescent light emission data from the surface portion of the animal.
An imaging system for obtaining a representation of a fluorescent probe distribution located inside an animal, the imaging system comprising:
- an imaging chamber that includes
- a set of walls that enclosing an interior cavity,
- a stage configured to support the animal within the interior cavity,
- a fluorescent excitation source, and
- a camera;
- and
- a processing system including a processor and memory, configured to:
- obtain one or more fluorescent images of at least a portion of the animal,
- obtain a three dimensional representation of a surface portion of the animal,
- map fluorescent image data from the one or more fluorescent images to the three dimensional representation of the surface portion of the animal to create fluorescent light emission data from the surface portion of the animal, and
- determine a three-dimensional representation of the fluorescent probe distribution internal to the animal using the fluorescent light emission data from the surface portion of the animal.
Dependencies are inferred from the claim text.