Raster microscope
AssigneeLeica Microsystems CMS GmbH
InventorHilmar Gugel
Life of this patent
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Abstract
A scanning microscope includes an excitation light beam, a stimulation light beam, an objective, an optical component and an optical system, The excitation light beam optically excites a first area of a specimen. The stimulation light beam triggers a stimulated emission or an additional excitation in a second area of the specimen, the second area at least partially overlapping with the first area of the specimen. The objective focuses the excitation light beam and the stimulation light beam. The optical component influences a shape of the focus of the excitation light beam and/or of the stimulation light beam. The optical system images the optical component into the pupil of the objective and adjusts a size of an image of the optical component.
Claims18
A scanning microscope comprising:
- an excitation light beam configured to optically excite a first area of a specimen;
- a stimulation light beam configured to trigger a stimulated emission or an additional excitation in a second area of the specimen, the second area at least partially overlapping with the first area of the specimen;
- an objective configured to focus the excitation light beam and the stimulation light beam;
- an optical component configured to influence a shape of a focus of at least one of the excitation light beam and the stimulation light beam;
- and
- an optical system configured to image the optical component into a pupil of the objective and to adjust a size of an image of the optical component while maintaining the image of the optical component in the pupil of the objective.
Dependencies are inferred from the claim text.