Method for adjusting confocal microscope
AssigneeYokogawa Electric Corporation
InventorsYasuhiro Tanibata, Kenji Hachiya
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Abstract
A method for adjusting a confocal microscope which includes a microscope unit and a confocal scanner unit of Nipkow disk type, emits a light beam for image measurement on a sample, images a returned fluorescent light of the light beam by a CCD (charge-coupled device) digital camera as a confocal image, and enables observation of the sample by the confocal image, the method includes matching a phase of rotation of the Nipkow disk with a phase of an exposure time of the CCD digital camera.
Claims14
A method for adjusting a confocal microscope which includes a microscope unit and a confocal scanner unit of Nipkow disk type, and images a returned fluorescent light of a light beam by a CCD (charge-coupled device) digital camera as a confocal image, the method comprising the step of:
- matching a phase of rotation of the Nipkow disk with a phase of an exposure time of the CCD digital camera,
- wherein a time (s) required to create one screen of the confocal image is obtained by the following equation:
- =s60/(a number of the rotation of the Nipkow disk*12),
- wherein a unit of the time (s) is a second.
Dependencies are inferred from the claim text.