Sample plate for glycoprotein analysis by MALDI mass spectrometry and preparation method of the same
AssigneeYangsun Kim
InventorsYangsun Kim, Jeong Heon Lee, Mi Young Ha, Ho Chul Jung, Hyoung Soon Park
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Abstract
The present invention relates to a sample plate for matrix-assisted laser desorption ionization (MALDI) mass spectrometry having a stable glyco-affinity layer of a carboxyalkyl boronic acid or carboxyaryl boronic acid and a preparation method of the sample plate. The sample plate shows excellent selectivity for glycoprotein, and can be easily and cost effectively prepared. The sample plate is useful for glycoprotein analysis by MALDI mass spectrometry.
Claims20
A sample plate for glycoprotein analysis by matrix-assisted laser desorption ionization (MALDI) mass spectrometry comprising:
- an electrically conductive substrate having at least one sampling area;
- a layer of organosilane having an amine reactive functional group formed in the sampling area;
- a polymeric multi-amine layer of a polyalkyleneimine formed on the organosilane layer;
- and
- a glyco-affinity layer of a carboxyalkyl boronic acid or carboxyaryl boronic acid formed on the polymeric multi-amine layer.
A method of preparing a sample plate for glycoprotein analysis by matrix-assisted laser desorption ionization (MALDI) mass spectrometry comprising the steps of:
- defining at least one sampling area on an electrically conductive substrate;
- forming an organosilane layer on the sampling area using an organosilane having an amine reactive functional group;
- forming a polymeric multi-amine layer on the organosilane layer using a polyalkyleneimine;
- and
- forming a glyco-affinity layer on the polymeric multi-amine layer using a carboxyalkyl boronic acid or carboxyaryl boronic acid.
Dependencies are inferred from the claim text.