Refined plant transformation
AssigneeJ.R. Simplot Company
InventorsJ. Troy Weeks, Caius Rommens
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Abstract
The present invention provides methods for producing transgenic plants based on an optimized transfer of DNA from Agrobacterium to plant cells, and/or on an optimized integration of the transferred DNAs into plant cell genomes. It also provides Agrobacterium-transformation vectors that can be used to limit or eliminate the transfer of undesirable DNA. The present invention can be applied to essentially any species of plants, including many recalcitrant plant species.
Claims61
A method for producing a transgenic plant, comprising (a) vortexing a solution comprising a germinating plant seedling and at least one Agrobacterium strain that harbors a plasmid vector carrying a desired polynucleotide for about 60 seconds to several hours;
- (b) cultivating said seedling to produce a plant by transferring the seedling to soil or to hormone-free medium and without inducing a callus phase;
- and (c) screening said plant to determine if said desired polynucleotide is integrated into the genome of at least one cell of said plant, wherein said plant is stably transformed, and wherein the step of agitating the solution does not comprise sonication.
A method for producing a transgenic plant, comprising (a) vortexing a solution comprising a germinating plant seedling and at least one Agrobacterium strain that harbors a vector carrying a desired polynucleotide;
- (b) transferring said Agrobacterium-transformed seedling to soil or to hormone-free medium, and exposing said transformed seedling to conditions that promote growth without inducing a callus phase;
- and (c) screening said plants to determine if said desired polynucleotide is integrated into the genome of at least one cell of said plant, wherein a plant comprising said desired polynucleotide in said genome is a transgenic plant.
A method for producing a transgenic plant, comprising (a) vortexing a solution comprising a germinating plant seedling and at least one Agrobacterium strain that harbors a vector carrying a desired polynucleotide;
- (b) cultivating said seedling in callus induction medium to produce callus;
- (c) cultivating said callus in shoot induction medium to stimulate shoot growth, and then producing a plant;
- and (d) screening said plant to determine if said desired polynucleotide is incorporated into the genome of at least one cell of said plant, wherein a plant comprising said desired polynucleotide in said genome is a transgenic plant.
Dependencies are inferred from the claim text.