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US 7,598,385 B2Granted
Asymmetric cyanine fluorescent dyes
AssigneeShenzhen Mindray Biomedical Electronics Co., Ltd.
InventorsXiaojun Peng, Tong Wu, Jiangli Fan, Shiguo Sun, Bingshuai Wang, Bing Xu, Jianhui Shao
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Abstract
The present disclosure provides the asymmetric cyanine fluorescent dyes of formula I in which X, n, R1, R2, R3, R4, R5 and Y− are as defined in the specification. The present disclosure also provides conjugates of the fluorescent dyes, methods for preparation thereof, compositions comprising the fluorescent dyes, and methods for staining biological samples using the fluorescent dyes and compositions thereof.
Claims19
A fluorescent dye comprising a compound having the following general structural formula I:
- wherein
- n is 1, 2 or 3;
- X is chosen from:
- C(CH3)2, O, S or Se;
- R1 and R2 are each independently chosen from H, C1-18alkyl, —C1-6alkyl-OR5 or halogen;
- R3 is independently chosen from:
- H, C1-18alkyl, OR5, —C1-6alkyl-OR5, COOR5, NO2, CN or halogen;
- R4 is C1-18alkyl, —C1-6alkyl-OR5, benzyl or halogen, said benzyl optionally substituted with one or more substituents independently chosen from:
- halogen, hydroxyl, sulfhydryl, cyano, nitro, alkyl, aryl, alkoxy, heterocyclyl, haloalkyl, amino, alkylamino, amido, or carboxyl;
- R5 is H or C1-18alkyl;
- and
- Y− is a counter ion;
- or derivatives and conjugates thereof.
10 more elements
Dependencies are inferred from the claim text.