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AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsJeffrey Schlom, Dennis L. Panicali
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We have discovered that by using a recombinant viral vector, preferably a pox virus vector having at least one insertion site containing a DNA segment encoding prostate-specific antigen (PSA), operably linked to a promoter capable of expression in the host, a specific humoral and cellular immune response to PSA can be generated. The method preferably comprises introducing a sufficient amount of the recombinant pox virus vector into a host to stimulate the immune response, and contacting the host with additional PSA at periodic intervals thereafter. The additional PSA may be added by using a second pox virus vector from a different pox genus. In another embodiment, additional PSA can be added by contacting the host with PSA by a variety of other methods, including in one preferred embodiment adding PSA. The PSA may be formulated with an adjuvant or in a liposomal formulation.
A method for generating a cytotoxic T-cell eliciting immune response to prostate-specific antigen (PSA) in a human host, comprising administering to the host a first pox virus vector to stimulate an immune response, wherein the first pox virus vector has at least one insertion site containing a DNA segment encoding PSA or a cytotoxic T-cell eliciting epitope thereof operably linked to a promoter such that the DNA segment is expressed to produce PSA or the cytotoxic T-cell eliciting epitope thereof in the host in a sufficient amount to generate a cytotoxic T-cell eliciting immune response, and then administering an additional PSA or T-cell eliciting epitope thereof in a manner selected from the group consisting of in a second pox virus vector, in a formulation with an adjuvant, with a cytokine, with a co-stimulatory molecule, in a liposomal formulation, and a combination thereof.
Dependencies are inferred from the claim text.