Process for solubilizing glucagon-like peptide 1 compounds
AssigneeEli Lilly and Company
InventorsWalter Francis Prouty, Jr., Joseph Vincent Rinella, Jr.
Life of this patent
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Abstract
Disclosed is a method of preparing a GLP-1 compound that is soluble in aqueous solution at pH 7.4 from a GLP-1 compound that is substantially insoluble in aqueous solution at pH 7.4. The insoluble GLP-1 compound is dissolved in aqueous base or in aqueous acid to form a GLP-1 solution. The GLP-1 solution is then neutralized to a pH at which substantially no amino acid racemization of the GLP-1 compounds occurs, after which the soluble GLP-1 compound is isolated from the neutralized solution.
Claims2
A method of preparing Val8-GLP-1(7-37)OH [SEQ ID NO:15] that is soluble in aqueous solution at physiological pH, said Val8-GLP-1(7-37)OH being referred to as “soluble Val8-GLP-1(7-37)OH”, from Val8-GLP-1(7-37)OH that is insoluble in aqueous solution at physiological pH, said Val8-GLP-1(7-37)OH being referred to as an “insoluble Val8-GLP-1(7-37)OH”, said method comprising the steps of:
- a) dissolving insoluble Val8-GLP-1(7-37)OH in aqueous base having a pH between about 12.1 to about 12.5 to form a Val8-GLP-1(7-37)OH solution;
- b) pH-adjusting the Val8-GLP-1(7-37)OH solution within about thirty minutes after dissolution of Val8-GLP-1(7-37)OH to a pH at which substantially no amino acid racemization of the dissolved Val 8-GLP-1(7-37)OH occurs, wherein the pH is between about 7.0 to about 8.5;
- c) filtering the pH-adjusted solution of step b) through a filter membrane having a porosity of between about 0.20 μm to about 0.25 μm;
- and
- d) isolating soluble Val8-GLP-1(7-37)OH from the filtered solution of step c).
Dependencies are inferred from the claim text.