Protein imprinted polymers with integrated emission sites
AssigneeThe Research Foundation of State University of New York
InventorFrank V. Bright
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Abstract
This invention provides protein or polypeptide imprinted polymers with integrated emission sites (PIPIES) for detecting the presence of a protein or polypeptide analyte comprising templated sites which are specific for the analyte. At or near the templated sites are selectively placed reporter molecules. A method is also disclosed for the preparation of the PIPIES and the use of these for the detection of analytes.
Claims28
A molecularly imprinted polymer for detecting the presence of an analyte selected from the group consisting of polypeptide, peptide and protein, comprising a xerogel or aerogel polymer matrix comprising:
- a plurality of templated sites with each of the plurality of templated sites being specific for the analyte and having one or more reporter molecules selectively attached at the templated site,
- wherein substantially all of the reporter molecules are present at the templated sites, and wherein upon binding of the specific polypeptide, peptide or protein to the templated site, a change in the absorbance and/or emission of the reporter is observed.
A method of preparing a molecularly imprinted polymer for selectively detecting an analyte selected from the group consisting of protein and polypeptide comprising the steps of:
- a) allowing polymerization of unpolymerized polymer components in the presence of analyte molecules to form a polymer matrix having templated sites, wherein each templated site has an analyte molecule bonded thereto;
- b) releasing the analyte molecules from the templated sites thereby forming templated sites which are specific for the analyte;
- c) separately preparing an analyte-[activable reporter] complex, wherein the activiable reporter is formed by covalently bonding an activable chemical residue to a reporter molecule;
- d) contacting the templated sites with the analyte-[activable reporter] complexes;
- e) activating the analyte-[activable reporter] complex to form analyte-[activated reporter] complex and thereby effect binding of the reporter portion of the analyte-[activated reporter] complex to the templated site;
- and
- f) releasing the analyte molecule from the analyte-[activated reporter] complex thereby providing a molecularly imprinted polymer having a templated site and a reporter molecule bound at or near the site, and wherein the templated site is specific for the analyte.
A molecularly imprinted polymer prepared by a process comprising the following steps:
- a) allowing polymerization of unpolymerized polymer components comprising polymerizable precursors in the presence of an analyte selected from the group consisting of polypeptide, peptide and protein molecules to form a polymer matrix having a plurality of templated sites, wherein each of the plurality of templated sites has an analyte molecule bonded thereto;
- b) releasing the analyte molecules from a templated site thereby forming a templated site which is specific for the analyte;
- c) preparing an analyte-[activable reporter] complex, wherein the activable reporter is formed by covalently bonding an activable chemical residue to a reporter molecule;
- d) contacting the templated site from b) with the analyte-[activable reporter] complex;
- e) activating the analyte-[activable reporter] complex to form analyte-[activated reporter] complex, and thereby effecting binding of reporter portion of the analyte-[activated reporter] complex to the templated site;
- and
- f) releasing the analyte molecule from the analyte-[activated reporter] complex to obtain a molecularly imprinted polymer.
Dependencies are inferred from the claim text.