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US 7,598,075 B2Granted
Apparatus and methods for producing and using high-density cells and products therefrom
AssigneeProtein Sciences Corporation
InventorsGale E. Smith, John Knell, Andrei I. Voznesensky
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Abstract
Disclosed and claimed is apparatus and methods for the growth of cells to high density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the Spodoptera frugiperda Sf900+ cell line (ATCC: CRL 12579). Further disclosed is the infection of Sf900+ cells at high cell density with wild type and recombinant baculoviruses to produce baculovirus and DNA or gene or expression products.
Claims6
An apparatus for growing cells comprising:
- (a) at least one bioreactor for cell culture;
- (b) at least one vessel for culture medium;
- whereby the bioreactor and vessel are in fluid communication, and
- wherein the bioreactor and/or vessel are optionally stirred;
- (c) a dialysis means for circulating culture medium and/or cell culture,
- whereby there is a first, cell culture loop between the bioreactor and the dialysis means and a second, media replenishment loop between the vessel and the dialysis means;
- (d) in operation dialysis between the culture medium and the cell culture;
- (e) at least one means for delivery of oxygen comprising a hollow fiber filter oxygenator,
- whereby the oxygen is delivered directly to cells in a circulating loop of cells before cell entry into the hollow fiber filter.
6 more elements
Dependencies are inferred from the claim text.