Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose
AssigneeWeyerhaeuser NR Company
InventorsPramod K. Gupta, Diane Gail Holmstrom, Bonnie Larson
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Abstract
The present invention provides methods for producing cotyledonary pine embryos. The methods of the invention each include the step of culturing embryogenic pine tissue in, or on, a medium comprising a disaccharide and glucose to yield cotyledonary pine embryos, wherein the disaccharide and glucose are each present in the medium at a concentration of less than 3 percent. The present invention also provides cotyledonary pine embryos prepared by a method of the invention.
Claims26
A method for producing cotyledonary pine embryos, said method comprising the steps of:
- (a) culturing embryogenic pine tissue in, or on, a maintenance medium comprising maltose as the sole source of sugar to promote multiplication of the embryogenic tissue;
- and
- (b) culturing the embryogenic pine tissue multiplied in accordance with step (a) in, or on, a development medium comprising maltose and glucose to yield cotyledonary pine embryos, wherein said maltose is present in the development medium at a concentration of 2.5% and said glucose is present in the development medium at a concentration of 1%.
A method for producing cotyledonary pine embryos, said method comprising the steps of:
- (a) culturing embryogenic pine tissue in, or on, a maintenance medium comprising maltose as the sole source of sugar to promote multiplication of the embryogenic tissue;
- and
- (b) culturing the embryogenic pine tissue multiplied in accordance with step (a) in, or on, a development medium comprising abscisic acid, an absorbent composition, PEG, maltose, and glucose to form cotyledonary pine embryos, wherein said maltose is present in the development medium at a concentration of 2.5%, said glucose is present in the development medium at a concentration of 1%.
Dependencies are inferred from the claim text.