Enzymatic hydrolysis of biomasses having a high dry matter (DM) content
AssigneeInbicon A/S
InventorsClaus Felby, Jan Larsen, Henning Jørgensen, Jakob Vibe-Pedersen
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Abstract
Disclosed are processes for liquefaction and saccharification of polysacharide containing biomasses having high dry matter content (>20%) and preferably possessing large average particle size. The polysaccharide containing biomasses are subject to enzymatic hydrolysis in a mixer that utilizes “free fall” mixing. “Free fall” mixing provides mechanical degradation of the biomass during hydrolysis and, in the case of lignocellulosic biomass, promotes movement of cellulase enzymes along cellulose chains, which improves enzymatic hydrolysis at high dry matter.
Claims20
A process for treating polysaccharide containing biomasses characterized by subjecting a polysaccharide containing biomass having a final dry matter content of above 20% and comprising lignocellulosic biomass to:
- a) enzymatic hydrolysis using at least one cellulase enzyme, and
- b) mixing by a free fall type mixing that provides mechanical degradation of the biomass during hydrolysis,
- wherein the mixed biomass is liquefied to a liquid without any or with only a few remaining large fibres and particles in the process.
A process for treating polysaccharide containing biomasses characterized by subjecting a polysaccharide containing biomass having a final dry matter content of above 20% and comprising lignocellulosic biomass to:
- a) enzymatic hydrolysis using at least one cellulase enzyme, and
- b) mixing by a free fall type mixing that provides mechanical degradation of the biomass during hydrolysis,
- wherein the biomass comprises a lignocellulosic biomass having a distribution of particle sizes in which at least 20% (w/w) are between 26-70 mm in size before pre-treatment, and
- wherein the mixed biomass is liquefied to a liquid without any or with only a few remaining large fibres and particles in the process.
A process for treating polysaccharide containing biomasses characterized by subjecting a polysaccharide containing biomass having a final dry matter content of above 20% and comprising lignocellulosic biomass to:
- a) enzymatic hydrolysis using at least one cellulase enzyme, and
- b) mixing by a free fall type mixing that provides mechanical degradation of the biomass during hydrolysis,
- wherein the mixed biomass is liquefied to a liquid without any or with only a few remaining large fibres and particles in the process within 3 to 24 hours.
Dependencies are inferred from the claim text.