Process for producing ethanol by using recombinant coryneform bacterium
AssigneeResearch Institute of Innovative Technology for the Earth
InventorHideaki Yukawa
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Abstract
The present invention provides a process for highly efficiently producing ethanol at a high productivity consisting of using a coryneform bacterium which has been transformed by a DNA containing a gene expressing pyruvate decarboxylase activity and, if desired, a gene expressing alcohol dehydrogenase activity under a regulatory sequence allowing for the expression, under ethanol production conditions wherein this bacterium does not substantially proliferate to produce ethanol.
Claims2
A process for producing ethanol in a coryneform bacterium selected from the group consisting of Corynebacterium glutamicum and Brevibacterium lactofermentum by using pyruvate produced from sugars via a metabolizing pathway in the coryneform bacterium, the process comprising:
- a) transforming the coryneform bacterium with a polynucleotide having the nucleic acid sequence of SEQ ID NO:
- 7, and
- b) culturing the transformed bacterium under anaerobic conditions in a medium containing 200 μg/l of biotin, wherein the transformed bacterium does not proliferate.
A process for producing ethanol in a coryneform bacterium comprising:
- a) transforming a coryneform bacterium selected from the group consisting of Corynebacterium glutamicum and Brevibacterium lactofermentum with a polynucleotide having the nucleic acid sequence of SEQ ID NO:
- 7,
- b) culturing the transformed coryneform bacterium under anaerobic conditions in a medium containing 200 μg/l of biotin, such that the transformed bacterium does not proliferate, and
- c) collecting the ethanol produced thereby.
Dependencies are inferred from the claim text.