Neublastin expression constructs
AssigneeBiogen Idec MA Inc.
InventorsNels E. Pederson, William P. Sisk
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Abstract
A method of producing a secreted neublastin polypeptide using a heterologous signal sequence is disclosed. The secreted neublastin does not contain a neublastin pro sequence.
Claims18
A method of making a secreted neublastin polypeptide, the method comprising:
- providing a eukaryotic host cell transformed with a DNA comprising a nucleotide sequence that encodes a secreted neublastin polypeptide lacking a functional neublastin signal peptide and a neublastin pro-domain, wherein the neublastin polypeptide is operatively linked to a signal sequence selected from the group consisting of rat albumin signal sequence and human growth hormone signal sequence, and
- culturing the eukaryotic cell under conditions so that the neublastin polypeptide is expressed and secreted.
A nucleic acid comprising a nucleotide sequence that encodes a secreted neublastin polypeptide lacking a functional neublastin signal peptide and a neublastin pro-domain, wherein the neublastin polypeptide is operatively linked to a signal sequence selected from the group consisting of a rat albumin signal sequence, the modified albumin signal sequence MKWVTFLLFLLFISGEAFA (amino acids 1 to 19 of SEQ ID NO:26), and a human growth hormone signal sequence.
Dependencies are inferred from the claim text.