Back to results
US 7,598,054 B2Granted
Rapid peptidoglycan-based assay for detection of bacterial contamination of platelets
AssigneeImmunetics, Inc.
InventorsVictor Kovalenko, Andrew E. Levin, Lee Anne Beausang
Life of this patent
Estimating the term…
Abstract
The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample.
Claims28
A method for detecting bacteria or bacterial fragments in a platelet containing sample, comprising the steps of:
- (a) incubating the sample with hemolymph from a Lepidopteran insect, a phenoloxidase substrate that generates a reaction product, and 3-methyl-2-benzothiazolinone hydrazone or other hydrazone derivative;
- and,
- (b) detecting the formation of color, wherein formation of color indicates the presence of bacteria or bacterial fragments in the sample and the absence of color indicates the absence of bacteria or bacterial fragments in the sample.
A method for detecting bacteria or bacterial fragments in a platelet containing sample, comprising the steps of:
- (a) extracting the sample in an extraction solution,
- (b) incubating the sample with hemolymph from a Lepidopteran insect, dopamine and 3-methyl-2-benzothiazolinone hydrazone or other hydrazone derivative dissolved in neutralization buffer, and
- (c) detecting the formation of color, wherein the formation of color indicates the presence of bacteria or bacterial fragments in the sample and the absence of color indicates the absence of bacteria or bacterial fragments in the sample.
Dependencies are inferred from the claim text.