Use of prion conversion modulating agents
AssigneeLaboratories Serono SA
InventorsClaudio Soto-Jara, Kinsey Maundrell
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Abstract
The use of Apolipoprotein B, Apolipoprotein E, fragments and mimetics thereof is provided for diagnostic, detection, prognostic and therapeutic applications In prion diseases. More specifically, the invention provides the use of Apolipoprotein B or fragments thereof for modulating or identifying modulators of the prion protein replication which are implicated in the pathogenesis of transmissible spongiform encephalopathics and other prion diseases.
Claims30
A method for the detection of a prion disease within a subject suspected of suffering from such a disease, the method comprising:
- (i) contacting a sample from said subject with Apolipoprotein B or a peptide fragment thereof;
- (ii) contacting the preparation obtained in step (i) with PrPC or a PrPC containing mixture;
- and
- (iii) determining the presence and/or an amount of PrPSc in said sample;
- wherein the presence of PrPSc in said sample is indicative of the presence of prions in said subject.
A method for the detection of PrPSc within a sample, comprising:
- (i) contacting said sample with Apolipoprotein B or a peptide fragment thereof;
- (ii) contacting the sample obtained in (i) with PrPC or a PrPC containing mixture;
- and
- (iii) determining the presence and/or an amount of PrPSc in said sample,
- wherein the presence of PrPSc indicates that the sample contains PrPSc.
A method for identifying, in a sample, a compound which modulates the transition of PrPC into PrPSc, comprising:
- (i) contacting said sample with Apolipoprotein B or a peptide fragment thereof;
- in the presence of said modulatory compound and (b) in the absence of said compound;
- (ii) contacting the preparation obtained in step (i) a and (i) b with PrPC or a PrPC containing mixture;
- and
- (iii) determining the amount of PrPSc (a) in the presence of said modulatory compound and (b) in the absence of said modulatory compound,
- wherein the presence of PrPSc identifies a compound that modulates the transition of PrPC into PrPSc.
A method for the detection of PrPSc in a biological sample comprising:
- (i) contacting a sample from a subject exposed to a prion with Apolipoprotein B or a peptide fragment thereof;
- (ii) contacting the preparation obtained in step (i) with PrPC or PrPC containing mixtures;
- and
- (iii) determining the presence and/or an amount of PrPSc in said sample,
- wherein the presence of PrPSc in said sample is indicative of the presence of prions in the sample.
Dependencies are inferred from the claim text.