Back to results
US 7,598,030 B2Granted
Antibodies against cancer produced using masked cancer cells as immunogen
AssigneeAlexion Pharmaceuticals, Inc.
InventorsKatherine S. Bowdish, Hong Xin, Toshiaki Maruyama, Naveen Dakappagari
Life of this patent
Estimating the term…
Abstract
This disclosure relates to methods for selecting antibodies having desirable characteristics from a population of diverse antibodies. More specifically, this disclosure provides methods for identifying antibodies which bind to cancer cells, but which do not bind to human red or white blood cells or normal tissue cells. Antibodies of the disclosure can be used for therapeutic and/or diagnostic purposes.
Claims13
A method comprising:
- collecting antiserum from non-human subjects immunized with non-cancerous human cells;
- contacting the antiserum with cancer cells to provide masked cancer cells;
- immunizing a subject with a composition comprising the masked cancer cells;
- and
- recovering antibodies that bind to the cancer cells from the subject,
- wherein the recovering step comprises
- a) generating an antibody library from subjects immunized with a composition comprising the masked cancer cells;
- b) removing from the library
- i) antibodies that bind to human red and white blood cells and
- ii) antibodies that bind to at least one other type of non-cancerous cell selected from the group consisting of endothelial cells, epithelial cells, smooth muscle cells, liver cells, lung cells, heart cells, kidney cells, intestine cells, stomach cells, bladder cells, spleen cells, pancreas cells, bone marrow cells, brain cells, thymus cells, prostate cells, ovary cells, testis cells and skin cells;
- and c) then recovering from the library antibodies that bind to the cancer cell.
8 more elements
A method comprising:
- collecting antiserum from non-human subjects immunized with non-cancerous human cells;
- contacting the antiserum with cancer cells to provide masked cancer cells;
- immunizing a subject with a composition comprising the masked cancer cells;
- and
- recovering antibodies that bind to the cancer cells from the subject,
- wherein the recovering step comprises
- a) collecting organs from subjects immunized with a composition comprising the masked cancer cells;
- b) generating hybridomas that express antibodies;
- and
- c) screening for antibodies that bind to the cancer cell but not to human red or white blood cells.
7 more elements
A method comprising:
- collecting antiserum from non-human subjects immunized with non-cancerous human cells;
- contacting the antiserum with cancer cells to provide masked cancer cells;
- immunizing a subject with a composition comprising the masked cancer cells;
- and
- recovering antibodies that bind to the cancer cells from the subject,
- wherein the recovering step comprises:
- a) generating a phage displayed antibody library using cells collected from subjects immunized with a composition comprising masked cancer cells;
- b) removing members of the library that bind to human red blood cells to generate a sub-library;
- and
- c) recovering from the sub-library members that display antibodies that bind to the cancer cell.
7 more elements
A method comprising:
- contacting cancer cells with one or more antibodies to receptors present on non-cancerous human cells to provide masked cancer cells;
- immunizing a subject with a composition comprising the masked cancer cells;
- and
- recovering antibodies that bind to the cancer cells from the subject,
- wherein the one or more antibodies to receptors known to be present on non-cancerous human cells are selected from the group consisting of complement receptors.
2 more elements
A method comprising:
- contacting cancer cells with one or more antibodies to receptors present on non-cancerous human cells to provide masked cancer cells;
- immunizing a subject with a composition comprising the masked cancer cells;
- and
- recovering antibodies that bind to the cancer cells from the subject,
- wherein the recovering step comprises
- a) generating an antibody library from subjects immunized with a composition comprising the masked cancer cells;
- b) removing from the library
- i) antibodies that bind to human red and white blood cells and
- ii) antibodies that bind to at least one other type of non-cancerous cell selected from the group consisting of endothelial cells, epithelial cells, smooth muscle cells, liver cells, lung cells, heart cells, kidney cells, intestine cells, stomach cells, bladder cells, spleen cells, pancreas cells, bone marrow cells, brain cells, thymus cells, prostate cells, ovary cells, testis cells and skin cells;
- and c) then recovering from the library antibodies that bind to the cancer cell.
7 more elements
A method comprising:
- contacting cancer cells with one or more antibodies to receptors present on non-cancerous human cells to provide masked cancer cells;
- immunizing a subject with a composition comprising the masked cancer cells;
- and
- recovering antibodies that bind to the cancer cells from the subject,
- wherein the recovering step comprises
- a) collecting organs from subjects immunized with a composition comprising the masked cancer cells;
- b) generating hybridomas that express antibodies;
- and
- c) screening for antibodies that bind to the cancer cell but not to human red or white blood cells.
6 more elements
A method comprising:
- contacting cancer cells with one or more antibodies to receptors present on non-cancerous human cells to provide masked cancer cells;
- immunizing a subject with a composition comprising the masked cancer cells;
- and
- recovering antibodies that bind to the cancer cells from the subject,
- wherein the recovering step comprises:
- a) generating a phage displayed antibody library using cells collected from subjects immunized with a composition comprising masked cancer cells;
- b) removing members of the library that bind to human red blood cells to generate a sub-library;
- and
- c) recovering from the sub-library members that display antibodies that bind to the cancer cell.
6 more elements
Dependencies are inferred from the claim text.