Metalloproteinase inhibitory agent
AssigneeNestec S.A.
InventorsMarcel-Alexandre Juillerat, Marie-Claude Robert
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Abstract
The present invention relates to an active agent, in particular a metalloproteinase inhibitory agent of casein-derived peptides obtained by hydrolysis of casein by a food grade bacteria. The invention also relates to the manufacture of an active agent, in which a food grade bacteria of the genus Lactobacillus helveticus is contacted with casein in order to perform a hydrolysis and obtain casein-derived peptides exhibiting metalloproteinases inhibitory property. The present invention also includes isolated and purified inhibitory peptides obtained by hydrolysis of casein by the bacteria.
Claims12
A metalloproteinases inhibitory agent comprising casein-derived peptides obtained by hydrolysis of casein by a food grade bacteria, wherein the agent is selected from the group consisting of isolated single-polypeptide-chain peptides set forth in SEQ ID Nos. 1, 3, 4, 5, 6 and 7, consisting of the amino acid sequence:
- (SEQ ID NO:
- 1)
- E-N-L-L-R-F-F-V-A-P-F-P-E-V-F,
- (SEQ ID NO:
- 3)
- N-E-N-L-L-R-F-F-V-A-P-F-P-E-V-F,
- (SEQ ID NO:
- 4)
- L-N-E-N-L-L-R-F-F-V-A-P-F-P-E-V,
- (SEQ ID NO:
- 5)
- E-N-L-L-R-F-F-V-A-P-F-P-E,
- (SEQ ID NO:
- 6)
- N-E-N-L-L-R-F-F-V-A-P-F-P-E,
- and
- (SEQ ID NO:
- 7)
- G-L-P-Q-E-V-L-N-E-N-L-L-R-F-F.
A process for the manufacture of a metalloproteinases inhibitory agent, the process comprising:
- preparing a solution comprising casein and a food grade bacteria, wherein the food grade bacteria comprises Lactobacillus helveticus CNCM I-2864,
- holding the solution under conditions effective to partially hydrolyze the casein to provide a metalloproteinases inhibitory agent containing casein-derived peptides, and
- stopping the hydrolysis by centrifugation to form the agent.
A process for the manufacture of a metalloproteinases inhibitory agent, the process comprising:
- preparing a solution comprising casein and a food grade bacteria, wherein the food grade bacteria comprises Lactobacillus helveticus CNCM I-2864 and a bacteria selected from the group consisting of Lactobacilli and Bacilli and combinations thereof,
- holding the solution under conditions effective to partially hydrolyze the casein to provide a metalloproteinases inhibitory agent containing casein-derived peptides, and
- stopping the hydrolysis by centrifugation to form the agent.
An inhibitor of metalloproteinases comprising a peptide selected from the group consisting of isolated single-polypeptide-chain peptides set forth in SEQ ID Nos. 1, 3, 4, 5, 6 and 7, consisting of the amino acid sequence:
- (SEQ ID NO:
- 1)
- E-N-L-L-R-F-F-V-A-P-F-P-E-V-F,
- (SEQ ID NO:
- 3)
- N-E-N-L-L-R-F-F-V-A-P-F-P-E-V-F,
- (SEQ ID NO:
- 4)
- L-N-E-N-L-L-R-F-F-V-A-P-F-P-E-V,
- (SEQ ID NO:
- 5)
- E-N-L-L-R-F-F-V-A-P-F-P-E,
- (SEQ ID NO:
- 6)
- N-E-N-L-L-R-F-F-V-A-P-F-P-E,
- and
- (SEQ ID NO:
- 7)
- G-L-P-Q-E-V-L-N-E-N-L-L-R-F-F.
Dependencies are inferred from the claim text.