Method of condensed cell slide preparation and detection of rarely occurring cells on microscope slides

A method for detection of a rarely occurring event within one or more biological samples comprising:

• a. processing each biological sample to provide a concentrated sample;
• b. depositing a concentrated sample in a gellable liquid solution on each of a plurality of slides;
• c. mounting the plurality of slides on a rotatable turntable in precisely identified locations;
• d. prescanning the plurality of slides using laser fluorescence excitation and collection to determine potential instances of the rarely occurring event within the concentrated sample;
• e. analyzing the potential instances of the rarely occurring event to filter out potential instances of the rarely occurring event from not actual instances of the rarely occurring event;
• f. maintaining location information for remaining potential instances of the rarely occurring event;
• g. using the location identification information to locate the potential instances of the rarely occurring event for additional analysis;
• and
• h. providing the additional analysis to detect instances of the rarely occurring event from the remaining potential instances.

A method for detection of a rarely occurring event within a biological sample comprising:

• providing a system comprising an automated cell dispensing tool for dispensing cells from the biological sample on microscope slides,
• a computer-controlled system for detection of rarely occurring cells on microscope slides using laser excited fluorescence collection;
• and
• processing each biological sample to provide a gellable liquid solution comprising concentrated biological sample and a flourochrome designed to associate with a rarely occurring event within the concentrated biological sample;
• depositing the solution in a layer on a surface of a slide using the automated cell dispensing tool;
• using the computer-controlled system for scanning the solution on the slide with energy adapted to cause fluorescence of the flourochrome to detect potential instances of the rarely occurring event within the concentrated biological sample in the solution.