Pectate lyases, nucleic encoding them and methods for making and using them

An isolated, synthetic or recombinant nucleic acid molecule comprising

• (a) a nucleic acid sequence having at least 95%, 96%, 97%, 98%, 99%, or more, or 100% sequence identity to SEQ ID NO:77 or SEQ ID NO:131 and encoding a polypeptide having pectate lyase (EC 4.2.2.2) activity, or enzymatically active fragments thereof;
• (b) a nucleic acid sequence comprising the sequence SEQ ID NO:77 or SEQ ID NO:131 and encoding a polypeptide having pectate lyase (EC 4.2.2.2) activity, or an enzymatically active fragment thereof;
• (c) a nucleic acid sequence encoding a polypeptide having pectate lyase (EC 4.2.2.2) activity, wherein the polypeptide has at least 95%, 96%, 97%, 98%, 99%, or more, or 100% sequence identity to SEQ ID NO:78 or SEQ ID NO:132, or an enzymatically active fragment thereof;
• (d) a nucleic acid sequence encoding a polypeptide having pectate lyase (EC 4.2.2.2) activity, wherein the polypeptide has the sequence SEQ ID NO:78 or SEQ ID NO:132, or an enzymatically active fragment thereof;
• (e) the nucleic acid sequence of (c) or (d) further comprising a heterologous sequence encoding a signal sequence, a catalytic domain, and/or prepro domain from a different pectate lyase operably linked to the nucleic acid sequence or encoding a signal sequence and/or prepro domain from a non-pectate lyase protein linked to the nucleic acid sequence;
• (f) a sequence complementary to the full-length nucleic acid of (a), (b), (c), (d), or (e).