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US 7,592,168 B2Granted
Cephalosporin C acylase mutant and method for preparing 7-ACA using same
AssigneeSandoz Ltd.
InventorsYong Chul Shin, John Y J Jeon, Kyung Hwa Jung, Mi Ran Park, Youngso Kim
Life of this patent
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Abstract
A CPC acylase mutant of the present invention has an improved reactivity and specific activity to CPC, which can be efficiently employed for directly preparing 7-ACA from CPC by a one-step enzymatic method.
Claims17
A CPC acylase mutant, wherein at least one amino acid selected from the group consisting of Val121α, Gly 139α and Phe 169α of CPC acylase α-subunit of SEQ ID NO:
- 4 and Phe 58β, His 70β, Ile 75β, Ile 176β and Ser 471β of CPC acylase β-subunit of SEQ ID NO:
- 5 is replaced by another amino acid.
Dependencies are inferred from the claim text.