Signal for packaging of influenza virus vector
AssigneeWisconsin Alumni Research Foundation
InventorYoshihiro Kawaoka
Life of this patent
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Abstract
The invention provides a packaging (incorporation) signal for influenza virus vectors, and methods of using the signal to transmit and maintain influenza viral and foreign nucleic acid in virus and cells.
Claims31
An influenza viral vector, comprising influenza virus incorporation sequences, which vector comprises:
- sequences corresponding to the 3′ noncoding region of influenza virus HA vRNA and HA coding sequences which include 3′ HA incorporation sequences, a nucleic acid segment for a heterologous open reading frame which encodes a gene product, and HA coding sequences which include 5′ HA incorporation sequences and the 5′ noncoding region of HA vRNA, wherein the HA coding sequences which include 3′ HA incorporation sequences include at least 3 nucleotides of 5′ HA coding sequence and the HA coding sequences which include the 5′ HA incorporation sequences have no more than 1011 nucleotides of 3′ HA coding sequences.
An isolated nucleic acid molecule comprising sequences corresponding to the 3′ noncoding region of influenza virus HA vRNA which include 3′ HA incorporation sequences, a nucleic acid segment for a heterologous open reading frame which encodes a gene product, and influenza virus 5′ HA incorporation sequences that include sequences corresponding to the 3′ coding region of HA, wherein the 3′ HA coding region is no more than 1011 nucleotides, and wherein the nucleic acid molecule does not include incorporation sequences corresponding to the 5′ HA coding region.
A vector comprising sequences corresponding to the 3′ noncoding region of influenza virus HA vRNA and HA coding sequences which include 3′ HA incorporation sequences, a nucleic acid segment for a heterologous open reading frame which encodes a gene product, and HA coding sequences which include 5′ HA incorporation sequences and the 5′ noncoding region of HA vRNA, wherein the HA coding sequences which include 3′ HA incorporation sequences include at least 3 nucleotides of 5′ HA coding sequence and the HA coding sequences which include the 5′ HA incorporation sequences have no more than 1011 nucleotides of 3′ HA coding sequences.
An influenza viral vector, comprising influenza virus incorporation sequences, which vector comprises:
- sequences corresponding to the 3′ noncoding region of influenza virus HA vRNA and HA coding sequences which include 3′ HA incorporation sequences, a nucleic acid segment for a heterologous open reading frame which encodes a gene product, and HA coding sequences which include 5′ HA incorporation sequences and the 5′ noncoding region of HA vRNA, wherein the HA coding sequences which include 3′ HA incorporation sequences include no more than 966 nucleotides of 5′ HA coding sequence and the HA coding sequences which include the 5′ HA incorporation sequences have at least 54 nucleotides of 3′ HA coding sequences.
A vector comprising sequences corresponding to the 3′ noncoding region of influenza virus HA vRNA and HA coding sequences which include 3′ HA incorporation sequences, a nucleic acid segment for a heterologous open reading frame which encodes a gene product, and HA coding sequences which include 5′ HA incorporation sequences and the 5′ noncoding region of HA vRNA, wherein the HA coding sequences which include 3′ HA incorporation sequences include no more than 966 nucleotides of 5′ HA coding sequence and the HA coding sequences which include the 5′ HA incorporation sequences have at least 54 nucleotides of 3′ HA coding sequences.
Dependencies are inferred from the claim text.