Surface enhanced raman spectroscopy (SERS) systems and methods of use thereof

A method of detecting at least one biomolecule in a sample, comprising:

• attaching at least one first biomolecule to an array of nanorods on a substrate, wherein a tilt angle (β) between an individual nanorod and the substrate surface is about 0° to about 90°;
• exposing the substrate including the first biomolecule to the sample containing at least one of a second biomolecule and a third biomolecule;
• and
• measuring a surface enhanced Raman spectroscopy (SERS) spectrum, wherein a SERS spectrum of the array of nanorods and the first biomolecule is detectably different than a SERS spectrum of the array of nanorods, the first biomolecule, and the second biomolecule and a SERS spectrum of the array of nanorods, the first biomolecule, and the third biomolecule, and wherein the SERS spectrum of the array of nanorods, the first biomolecule, and the second biomolecule is detectably different than the SERS spectrum of the array of nanorods, the first biomolecule, and the third biomolecule.