Ion source for electron transfer dissociation and deprotonation
AssigneeBruker Daltonik GmbH
InventorRalf Hartmer
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Abstract
In mass spectrometric analysis equipment, highly charged analyte fragment ions are obtained by electron transfer dissociation of highly charged analyte ions by radical anions and the dissociation products are then deprotonated with non-radical anions. Both the radical anions for electron transfer dissociation and the non-radical anions for deprotonation are produced in a single ion source from a single substance, or a single mixture of substances by adjusting the electrical operating parameters of the ion source.
Claims20
A method for measuring the mass spectra of fragment ions using electron transfer dissociation of analyte ions by means of reactions with radical anions and also partial deprotonation of the analyte ions or the fragment ions by means of reactions with non-radical anions, wherein both the radical anions for electron transfer dissociation and the non-radical anions for deprotonation are created in the same ion source from the same substance or the same mixture of substances.
A method for measuring a spectrum of partially deprotonated fragment ions produced from ions of an analyte substance, the latter ions having multiple positive charges that have been created by electron transfer dissociation in a mass spectrometer with an RF ion trap, comprising:
- a) creating positively charged analyte ions from the analyte substance, and storing the analyte ions in the RF ion trap;
- b) producing radical anions and non-radical anions from a single reactant substance or a single mixture of reactant substances within a single electron attachment ion source and providing the radical anions and the non-radical anions to the RF ion trap for electron transfer dissociation and for deprotonation, respectively, and
- c) measuring the fragment ion spectrum.
Dependencies are inferred from the claim text.