Methods and kits for detecting SARS-associated coronavirus

A primer set for determining the presence or absence of SARS-associated corona virus in a biological sample, wherein the primer set comprises at least one synthetic nucleic acid sequence selected from the group consisting of:

• (a) a synthetic nucleic acid sequence consisting of from 19 to 30 consecutive nucleotides of SEQ ID NO:
• 43;
• and
• (b) a synthetic nucleic acid sequence consisting of from 19 to 30 consecutive nucleotides of a nucleic acid sequence that is complementary to SEQ ID NO:
• 43.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is the nucleic acid of SEQ ID NO:
• 2,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 3,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 4,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 5,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 6,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ IDNO:
• 7,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 8,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 9,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 10,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 11,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 12,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 13,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 14,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 15,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.

A method for determining the presence or absence of SARS-associated corona virus in a sample, the method comprising:

• a) contacting nucleic acid from a sample with at least one primer which is a nucleic acid of SEQ ID NO:
• 16,
• b) subjecting the nucleic acid and the primer to amplification conditions, and
• c) determining the presence or absence of amplification product.