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AssigneeChildren's Hospital Medical Center
InventorsKejian Zhang, Richard J. Wenstrup, Alexandra H. Filipovich
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The methods and compositions of the invention find use in the clinical diagnosis of primary immunodeficiencies, particularly Wiskott-Aldrich related syndromes. The compositions of the invention include isolated nucleic acid molecules and oligonucleotide pairs suitable for use in amplifying regions of the Wiskott-Aldrich syndrome protein gene and in determining the nucleotide sequence of the Wiskott-Aldrich syndrome protein gene in a patient. The invention facilitates efficient, cost-effective amplification of one or more regions of the Wiskott-Aldrich syndrome protein gene. The nucleotide sequence of amplified DNA comprising one or more regions of the Wiskott-Aldrich Syndrome Protein gene can be determined using the methods and compositions of the invention. Knowledge of the patient's nucleotide sequence in the Wiskott-Aldrich Syndrome Protein gene allows diagnosis of the patient's primary immunodeficiency.
An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of:
An oligonucleotide pair comprising a first nucleic acid molecule and a second nucleic acid molecule, wherein said first nucleic acid molecule consisting of the nucleotide sequence set forth in SEQ ID No:
A kit for performing a method of amplifying a region of the Wiskott-Aldrich syndrome protein gene comprising an oligonucleotide pair consisting of:
Dependencies are inferred from the claim text.