Vectors and methods for high throughput co-expression

A polynucleotide comprising a first nucleotide sequence operably encoding tetracycline resistance and a second nucleotide sequence operably encoding a ccdB polypeptide, wherein the first nucleotide sequence and the second nucleotide sequence are flanked within the same attR3 and attR4 sites, and wherein the polynucleotide comprises a tet Multisite having SEQ ID NO:

• 7.

An RNAi vector selected from the group consisting of pRIPPER-1 (SEQ ID NO:11), pRIPPER-2 (SEQ ID NO:12), pRIPPER-3 (SEQ ID NO:10), pRIPPER-4 (SEQ ID NO:13), pRIPPER-II (SEQ ID NO:14), pRIPPER-III (SEQ ID NO:15), and pRIPPER-IV (SEQ ID NO:16).

An RNA interference (RNAi) vector comprising a polynucleotide cassette, the polynucleotide cassette comprising a nucleotide sequence operably encoding zeomycin resistance and a nucleotide sequence operably encoding a ccdB polypeptide, wherein the polynucleotide cassette is flanked by attR1 and attR2 sites, and wherein the polynucleotide cassette comprises a G144704 cassette having SEQ ID NO:

• 4.

An RNA interference (RNAi) vector comprising a polynucleotide cassette, the polynucleotide cassette comprising a nucleotide sequence operably encoding tetracycline resistance and a nucleotide sequence operably encoding a ccdB polypeptide, wherein the polynucleotide cassette is flanked by attR3 and attR4 sites, and wherein the polynucleotide cassette flanked by attR3 and attR4 sites comprises a tet Multisite having SEQ ID NO:

• 7.