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US 7,582,470 B2Granted
Device for amplifying and detecting a target nucleic acid
AssigneeGen-Probe Incorporated
InventorKenneth A. Browne
Life of this patent
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Abstract
Compositions, methods and devices for detecting nucleic acids. The invention particularly regards composite arrays of immobilized amplification primers and hybridization probes. Also disclosed are compositions and methods for covalently immobilizing oligonucleotides and other biological molecules to glass and plastic surfaces.
Claims10
A device for amplifying and detecting a target nucleic acid, comprising:
- a solid support bead having a surface;
- an amplification primer immobilized to the surface of said solid support bead, said amplification primer comprising a promoter sequence for an RNA polymerase and a sequence complementary to a first strand of said target nucleic acid;
- and
- a labeled hybridization probe separate from the amplification primer immobilized to said surface,
- wherein said labeled hybridization probe comprises 2′-methoxy nucleotide analogs and a sequence complementary to an amplicon synthesized using said amplification primer and said target nucleic acid as a template in a nucleic acid amplification reaction, and
- wherein prior to contact of said device with any nucleotide polymerizing enzyme said labeled hybridization probe comprises a detectable label and is immobilized to said surface.
3 more elements
Dependencies are inferred from the claim text.