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AssigneeBristol-Myers Squibb Company
InventorsNigel Ian Bowers, Paul M. Skonezny, Gregory L. Stein, Thomas Franceschini, Shu-Jen Chiang, Wendy L. Anderson, Li You, Zizhuo Xing
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The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648.
A process comprising preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane of the formula:
A process comprising preparing (2R,3S)-1-chloro-2-hydroxy-3-N-(tert-butoxycarbonyl)amino-4-phenylbutane by contacting Rhodococcus eryibropolis ATCC PTA-6648 with from about 4.5 to about 6% w/v, based on substrate, (3S)-1-chloro-2-oxo-3-N-(tert-butoxycarbonyl)amino-4-phenylbutane substrate;
Dependencies are inferred from the claim text.