Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsTakuo Yamamoto, Kazuhiko Maruta, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Abstract
A non-reducing saccharide-forming enzyme and a trehalose-releasing enzyme, which have an optimum temperature in a medium temperature range, i.e., a temperature of over 40 or 45° C. but below 60° C.; and an optimum pH in an acid pH range, i.e., a pH of less than 7. The two-types of enzymes can be obtained in a desired amount, for example, by culturing in a nutrient culture medium microorganisms capable of producing the enzymes or by recombinant DNA technology.
Claims13
A purified trehalose-releasing enzyme which hydrolyzes a non-reducing saccharide having a trehalose structure as an end unit to release said trehalose end unit from the rest of said non-reducing saccharide and which has an optimum temperature of over 45° C. but below 60° C., wherein said enzyme comprises the amino acid sequence of SEQ ID NO:9.
A purified trehalose-releasing enzyme which hydrolyzes a non-reducing saccharide having a trehalose structure as an end unit to release said trehalose end unit from the rest of said non-reducing saccharide and which has an optimum temperature of over 45° C. but below 60° C., wherein said enzyme is a fragment of an enzyme consisting of the amino acid sequence of SEQ ID NO:9.
Dependencies are inferred from the claim text.