Kinase and phosphatase assays
AssigneeLife Technologies Corporation
InventorsElizabeth A. Werner, Tony A. Klink, Jane A. Beebe, David A. Lasky, Karen M. Kleman-Leyer, Richard Somberg
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Abstract
Compositions, methods, and kits for detecting and monitoring kinase or phosphatase activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase or phosphatase alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase or phosphatase activity are also described.
Claims42
A composition of matter comprising:
- a) a peptide having a length from five to fifty amino acids and comprising a recognition motif for a serine/threonine kinase selected from the group consisting of PRAFSVG (SEQ ID NO:
- 56) and RRQFSLR (SEQ ID NO:
- 57);
- and
- b) a first detectable moiety,
- wherein the first detectable moiety is attached to the peptide.
A composition of matter comprising:
- a) a peptide having a length from five to fifty amino acids and comprising a recognition motif for a serine/threonine kinase selected from the group consisting of PRAFSVG (SEQ ID NO:
- 56) and RRQFSLR (SEQ ID NO:
- 57);
- and
- b) a first detectable moiety;
- and
- c) a second detectable moiety;
- wherein the first detectable moiety is attached to the peptide.
A composition of matter comprising:
- a) a peptide having a length from five to fifty amino acids and comprising a protein phosphatase recognition motif selected from the group consisting of PRAFS*VG (SEQ ID NO:
- 106) and RRQFS*LR (SEQ ID NO:
- 107), wherein S* represents a phosphorylated serine;
- and
- b) a first detectable moiety,
- wherein the first detectable moiety is attached to the peptide.
A composition of matter comprising:
- a) a peptide having a length from five to fifty amino acids and comprising a protein phosphatase recognition motif selected from the group consisting of PRAFS*VG (SEQ ID NO:
- 106) and RRQFS*LR
- (SEQ ID NO:
- 107), wherein S* represents a phosphorylated serine;
- b) a first detectable moiety,
- wherein the first detectable moiety is attached to the peptide;
- and
- c) a second detectable moiety.
Dependencies are inferred from the claim text.