3-phosphoglycerate dehydrogenase variants whose inhibition by L-serine is reduced, and genes encoding them
AssigneeWacker Chemie AG
InventorsThomas Maier, Renate Flinspach
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Abstract
A 3-phosphoglycerate dehydrogenase (PGD) which exhibits a susceptibility to inhibition by serine which is reduced a as compared with that of an Escherichia coli wild-type PGD and which possesses an amino acid sequence which differs from the amino acid sequence of the Escherichia coli wild-type PGD (SEQ ID NO: 2) in that an amino acid apart from glycine is present at position 249 or an amino acid apart from threonine is present at position 372.
Claims2
An isolated 3-phosphoglycerate dehydrogenase (PGD) which exhibits a susceptibility to inhibition by serine, the inhibition by serine being reduced as compared with that of an Escherichia coli wild-type PGD and which possesses an amino acid sequence which differs from the amino acid sequence of the Escherichia coli wild-type PGD of SEQ ID NO:
- 2 solely in that an amino acid selected from the group consisting of I, D, Y, G, S, E, R, K, P, H, W, F, A, N, Q, V, L, M and C is present at position 372 of SEQ ID NO. 2.
An isolated 3-phosphoglycerate dehydrogenase which exhibits a susceptibility to inhibition by serine, the inhibition by serine being reduced as compared with that of an Escherichia coli wild-type PGD and which possesses an amino acid sequence which differs from the amino acid sequence of the Escherichia coli wild-type PGD of SEQ ID NO:
- 2 solely in that,
- the amino acid D is present at position 349 of SEQ ID NO:
- 2 and the amino acid I is present at position 372 of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.