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US 7,582,459 B2Granted
Process for producing a capsular polysaccharide for use in conjugate vaccines
AssigneeDe Staat der Nederlanden, vert. door de minister van VWS
InventorsAhd Hamidi, Michel Francois Beurret
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Abstract
A method for producing a polysaccharide and a conjugate vaccine including the polysaccharide produced according to the method. A characteristic step in the method is that the pH of the culture medium is kept at a constant value with base or acid until adjustment with respectively base or acid is not possible anymore. Using the method, capsular polysaccharide may be obtained in a high yield in a relatively short time. The method is straightforward, reproducible and cost-effective.
Claims9
A method for recovering a polysaccharide from a fermentation broth, comprising:
- employing a cationic detergent to precipitate the polysaccharide or part of the contaminants from the supernatant to obtain a first polysaccharide fraction;
- employing alcohol to precipitate the polysaccharide from the first polysaccharide fraction to obtain a second polysaccharide fraction;
- subjecting the second polysaccharide fraction to an alcohol precipitation in the presence of an anionic detergent, whereby the alcohol is present in a concentration which is below a concentration at which the polysaccharide precipitates;
- precipitating the polysaccharide from the soluble fraction employing alcohol to obtain a polysaccharide precipitate;
- and
- dissolving the polysaccharide precipitate and subjecting it to concentration and diafiltration.
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