5-substituted hydantoin racemase, DNA coding for the racemase, and processes for producing optically active amino acids
AssigneeAjinomoto Co., Inc.
InventorsShunichi Suzuki, Norimasa Onishi, Kenzo Yokozeki
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Abstract
The present invention relates to a 5-substituted hydantoin racemase, which efficiently catalyzes racemization reactions at a high optimum temperature for racemization reactions, DNA coding for the racemase, and processes for producing optically active amino acids.
Claims17
A method of producing optically active amino acids, comprising contacting a 5-substituted hydantoin with an isolated polypeptide that is at least 95% identical to the amino acid sequence of SEQ ID NO:
- 2, wherein said isolated polypeptide has 5-substituted hydantoin racemase activity, and an isolated hydantoinase to produce a N-carbamyl amino acid;
- and contacting said N-carbamyl amino acid with an isolated carbamyl amino acid hydrolase.
A method of producing optically active amino acids, comprising contacting a 5-substituted hydantoin with an isolated 5-substituted hydantoin racemase, which racemizes 5-substituted hydantoin and consists of the amino acid sequence of SEQ ID NO:
- 2, wherein one to ten amino acid residues have been replaced, deleted, inserted, or added, and an isolated hydantoinase to produce a N-carbamyl amino acid;
- and contacting said N-carbamyl amino acid with an isolated carbamyl amino acid hydrolase.
Dependencies are inferred from the claim text.