Corn event PV-ZMGT32(nk603) and compositions and methods for detection thereof
AssigneeMonsanto Technology LLC
InventorsCarl Frederick Behr, Gregory R. Heck, Catherine Hironaka Hironaka, Jinsong You
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Abstract
The present invention provides a DNA construct that confers tolerance to transgenic corn plant. Also provided are assays for detecting the presence of the PV-ZMGT32(nk603) corn event based on the DNA sequence of the recombinant construct inserted into the corn genome and of genomic sequences flanking the insertion site.
Claims8
A recombinant DNA molecule comprising a DNA molecule selected from the group consisting of SEQ ID NO:7 and SEQ ID NO:8.
An isolated primer pair of DNA molecules, wherein a first primer of said primer pair comprises at least 11 contiguous nucleotides from nucleotide 1 to 686 of SEQ ID NO:8 or full complements thereof, and a second primer of said primer pair comprises at least 11 contiguous nucleotides from nucleotides 687 to 1183 of SEQ ID NO:8 or full complements thereof, and the pair of DNA molecules when used together in a DNA amplification method produces a diagnostic amplicon comprising SEQ ID NO:
- 11 or full complements thereof for corn event PV-ZMGT32(nk603) or progeny thereof.
A method of detecting the presence of SEQ ID NO:7 or SEQ ID NO:8, said sequences being diagnostic of event PV-ZMGT32, the method comprising the steps of:
- (a) obtaining a DNA sample from a corn plant or parts thereof;
- (b) contacting the DNA sample with a DNA primer pair;
- comprising DNA primer molecules comprising at least 11 contiguous nucleotides of SEQ ID NO:7 or its complement, or at least 11 contiguous nucleotides of SEQ ID NO:8 or its complement;
- (c) providing a nucleic acid amplification reaction condition;
- (d) performing said nucleic acid amplification reaction, thereby producing a DNA amplicon molecule comprising a DNA molecule selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12;
- and
- (e) detecting the DNA amplicon molecule.
A DNA molecule formed by the junction of an inserted heterologous nucleic acid molecule and genomic region of a maize plant, plant cell, or seed wherein said DNA molecule comprises a DNA molecule selected from the group consisting of SEQ ID NO:7 and SEQ ID NO:8.
Dependencies are inferred from the claim text.