Modified carbon and germanium surfaces
AssigneeWisconsin Alumni Research Foundation
InventorsTodd C. Strother, Lloyd M. Smith, Robert J. Hamers
Life of this patent
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Abstract
Chemically-modified surfaces on unoxidized carbon, silicon, and germanium substrates are disclosed. Ultraviolet radiation mediates the reaction of protected ω-modified, α-unsaturated aminoalkenes (preferred) with hydrogen-terminated carbon, silicon, or germanium surfaces. Removal of the protecting group yields an aminoalkane-modified silicon surface These amino groups can be coupled to terminal-modified oligonucleotides using a bifunctional crosslinker, thereby permitting the preparation of modified surfaces and arrays. Methods for controlling the surface density of molecules attached to the substrate are also disclosed.
Claims16
A method of creating modified carbon and germanium surfaces comprising:
- (a) reacting a straight, branched, or cyclic aminoalkene, carboxyalkene, or thioalkene with an unoxidized carbon or germanium substrate, wherein the amino, carboxy, or thio substituent on the alkene is protected, and wherein the reaction is photoinitiated, to yield a modified surface having bonded directly thereto, in the absence of any intervening oxygen atoms, substituted alkyl moieties.
A method of creating modified carbon and germanium surfaces comprising:
- (a) reacting t-BOC-protected 10-aminodec-1-ene with an unoxidized carbon or germanium substrate, wherein the reaction is photoinitiated, to yield a modified surface having bonded directly thereto, in the absence of any intervening oxygen atoms, substituted alkyl moieties.
A method of creating modified surfaces on unoxidized carbon and germanium surfaces comprising:
- (a) reacting an amino-, carboxy, or thiol-modified and protected alkene with an unoxidized carbon, or germanium substrate to yield a surface of protected, amino-, carboxy, or thiol-modified alkane molecules covalently bonded to the substrate in the absence of any intervening oxygen molecules;
- then
- (b) deprotecting the modified alkane molecules of step (a), thereby yielding a surface of unprotected modified alkane molecules bonded to the substrate;
- then
- (c) reacting the deprotected modified alkane molecules of step (b) with a crosslinker, whereby the crosslinker is attached to the modified alkane molecules;
- and then
- (d) attaching molecules to the crosslinker of step (c), thereby yielding a surface of molecules immobilized on the substrate.
A method of creating modified carbon and germanium surfaces comprising:
- (a) reacting a straight, branched, or cyclic protected carboxyalkene with an unoxidized carbon or germanium substrate, wherein the reaction is photoinitiated, to yield a modified surface having bonded directly thereto, in the absence of any intervening oxygen atoms, substituted alkyl moieties.
A method of creating modified carbon and germanium surfaces comprising:
- (a) reacting a straight, branched, or cyclic protected thioalkene with an unoxidized carbon or germanium substrate, wherein the reaction is photoinitiated, to yield a modified surface having bonded directly thereto, in the absence of any intervening oxygen atoms, substituted alkyl moieties.
A method of creating modified carbon and germanium surfaces comprising:
- (a) reacting a straight, branched, or cyclic substituted α-olefin selected from the group consisting of aminoalkenes, carboxyalkenes, and thioalkenes, wherein the substituent is bonded to an ω carbon of the alkene.
A method of creating modified carbon and germanium surfaces comprising:
- (a) reacting a straight, branched, or cyclic substituted alkene selected from the group consisting of aminoalkenes, carboxyalkenes, and thioalkenes with an unoxidized carbon or germanium substrate, wherein the reaction is photoinitiated, to yield a modified surface having bonded directly thereto, in the absence of any intervening oxygen atoms, substituted alkyl moieties;
- and then
- (b) attaching molecules to the substituted alkyl moieties bonded to the substrate, thereby yielding a modified surface having the molecules immobilized thereon.
Dependencies are inferred from the claim text.