Anti-idiotypic antibodies against factor VIII inhibitor and uses thereof
AssigneeD. Collen Research Foundation vzw
InventorsJean Guy G. Gilles, Jean-Marie R. Saint-Remy, Marc G. Jacquemin
Life of this patent
Estimating the term…
Abstract
The present invention discloses anti-idiotypic antibodies and fragments thereof against inhibitory Factor VIII anti-bodies, said inhibitory antibodies having an affinity for the C2 domain of Factor VIII. The anti-idiotypic antibodies of the present invention are able to completely neutralise in vitro and in an in vivo mouse model the inhibitory activity of FVIII inhibitors. The anti idiotypic antibodies of the present invention can be applied for the prevention, treatment or reduction of bleeding disorders of hemophilia patients with inhibitory antibody against the C2 domain of Factor VIII.
Claims20
A monoclonal anti-idiotypic antibody capable of neutralising a human Factor VIII inhibitory antibody, the said inhibitory antibody being directed towards the C2 domain of Factor VIII, characterized by the fact that the complementary determining regions CDR1, CDR2, and CDR3 of the variable heavy chains of said anti-idiotypic antibody have amino acid sequences depicted in SEQ ID NO:
- 5, SEQ ID NO:
- 6, and SEQ ID NO:
- 7, respectively and the complementary determining regions CDR1, CDR2, and CDR3 of the variable light chains of said anti-idiotypic antibody have amino acid sequences depicted in SEQ ID NO:
- 8, SEQ ID NO:
- 9 and SEQ ID NO:
- 10, respectively.
A monoclonal anti-idiotypic antibody capable of neutralising a human Factor VIII inhibitory antibody, the said inhibitory antibody being directed towards the C2 domain of Factor VIII, which is the monoclonal antibody Ab14C12 produced by the cell line 14C12 deposited at Belgian Coordinated Collection of Micro-organisms (BCCM) with Accession Number LMBP 5878CB or an antibody fragment derived therefrom, wherein said antibody fragment is capable of neutralizing said inhibitory antibody.
A monoclonal cell line, expressing an anti-idiotypic antibody capable of neutralising a human Factor VIII inhibitory antibody, the said inhibitory antibody being directed towards the C2 domain of Factor VIII, which is the cell line 14C12 deposited at BCCM with Accession Number LMBP 5878CB.
A monoclonal anti-idiotypic antibody capable of neutralising a human Factor VIII inhibitory antibody, the said inhibitory antibody being directed towards the C2 domain of Factor VIII, characterized by the fact that the heavy chain variable region of said anti-idiotypic antibody comprises the amino acid sequence depicted in SEQ ID NO:
- 2.
A monoclonal anti-idiotypic antibody capable of neutralising a human Factor VIII inhibitory antibody, the said inhibitory antibody being directed towards the C2 domain of Factor VIII, characterized by the fact that the light chain variable region of said anti-idiotypic antibody comprises the amino acid sequence depicted in SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.