Zwitterionic dyes for labeling in proteomic and other biological analyses
AssigneeMontana State University
InventorsEdward A. Dratz, Paul A. Grieco
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Abstract
The invention relates to compositions and methods useful in the labeling and identification of proteins. The invention provides for highly soluble zwitterionic dye molecules where the dyes and associated side groups are non-titratable and maintain their net zwitterionic character over a broad pH range, for example, between pH 3 and 12. These dye molecules find utility in a variety of applications, including use in the field of proteomics.
Claims25
An optical labeling molecule for labeling a target analyte comprising:
- a. a boron difluoride diaza-indacene-propionic acid (BODIPY) zwitterionic dye moiety comprising at least one positive or at least one negative charge moiety added to obtain a net neutral charge, wherein the positive charge moiety is selected from a group consisting of a quaternary ammonium group and a guanidinium group, wherein the positive charge moiety is not titratable between the pH of 3-12, and wherein the negative charge moiety is selected from the group consisting of a sulfonate group and a sulfate group, wherein the negative charge moiety is not titratable between the pH of 3-12;
- b. a functional linker moiety;
- and
- c. a titratable group moiety, wherein the titratable group is a tertiary amine which closely approximates the pK of the group removed from the target analyte by reaction with the functional linker.
The optical labeling molecule having formula (I):
The optical labeling molecule which is selected from the group consisting of:
Dependencies are inferred from the claim text.