Polynucleotides encoding novel PCSK9 variants
AssigneeBristol-Myers Squibb Company
InventorsGabriel A. Mintier, Jian Chen, Bowman Miao, Rex Arnold Parker
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Abstract
The present invention provides novel polynucleotides encoding PCSK9b and PCSK9c polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel PCSK9b and PCSK9c polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.
Claims22
An isolated nucleic acid molecule comprising a polynucleotide sequence selected from the group consisting of:
- (a) an isolated polynucleotide encoding a polypeptide comprising amino acids 1 to 315 of SEQ ID NO:2;
- and
- (b) an isolated polynucleotide encoding a polypeptide comprising amino acids 2 to 315 of SEQ ID NO:2.
An isolated nucleic acid molecule comprising the cDNA clone contained in plasmid PCSK9-b in ATCC Deposit No. PTA-7622.
An isolated polynucleotide consisting of a polynucleotide sequence encoding amino acids 10 to 256 of SEQ ID NO:2, wherein said polynucleotide encodes a polypeptide that is capable of lowering the level of LDL uptake in mammalian cells in which said polypeptide is recombinately expressed.
An isolated polynucleotide comprising a polynucleotide sequence encoding amino acids 1 to 279 of SEQ ID NO:2, wherein said polynucleotide encodes a polypeptide that is capable of lowering the level of LDL uptake in mammalian cells in which said polypeptide is recombinately expressed.
An isolated polynucleotide comprising a polynucleotide sequence encoding amino acids 37 to 315 of SEQ ID NO:2, wherein said polynucleotide encodes a polypeptide that is capable of lowering the level of LDL uptake in mammalian cells in which said polypeptide is recombinately expressed.
An isolated polynucleotide comprising a polynucleotide sequence encoding amino acids 2 to 284 of SEQ ID NO:2, wherein said polynucleotide encodes a polypeptide that is capable of lowering the level of LDL uptake in mammalian cells in which said polypeptide is recombinately expressed.
An isolated polynucleotide comprising a polynucleotide sequence encoding amino acids 16 to 315 of SEQ ID NO:2, wherein said polynucleotide encodes a polypeptide that is capable of lowering the level of LDL uptake in mammalian cells in which said polypeptide is recombinately expressed.
Dependencies are inferred from the claim text.